下调RAD54B表达可抑制肺癌细胞增殖，诱导细胞凋亡

摘要：2019年12月，贵州省人民医院胸外科(Department of Thoracic Surgery, Guizhou Provincial People’s Hospital, Guiyang, Guizhou Province, China) Di Liu

2019年12月，贵州省人民医院胸外科(Department of Thoracic Surgery, Guizhou Provincial People’s Hospital, Guiyang, Guizhou Province, China) Di Liu老师研究团队在《Journal of International Medical Research》上发表论文：

“Knockdown of RAD54B expression reduces cell proliferation and induces apoptosis in lung cancer cells”

“下调RAD54B表达可抑制肺癌细胞增殖，诱导细胞凋亡”

Abstract：

Objective: RAD54 homolog B (RAD54B), a member of the SNF2/SWI2 superfamily, is implicated in homologous recombination, and high RAD54B expression predicts the prognostic outcomes of lung adenocarcinoma. However, its role in lung carcinogenesis was unclear so this was determined in the present study.

Methods: We evaluated the gene and protein expression of RAD54B in 15 lung adenocarcinoma tissues and matched adjacent healthy lung tissues by real-time PCR, immunohistochemical staining, and western blotting. A549 lung cancer cells were transduced with lentivirus carrying small hairpin RNA (shRNA) against RAD54B (shRAD54B) or control shRNA (shCtrl), and cell proliferation, viability, apoptosis, and caspase 3/7 activity were evaluated.

Results: RAD54B protein expression was significantly higher in lung adenocarcinoma tissues than in healthy lung tissues. RAD54B gene expression was high in A549 cells but was efficiently knocked down using shRAD54B with an infection efficiency of 80% and a knockdown ratio of 72.2% compared with shCtrl. Suppressing RAD54B expression in A549 cells significantly reduced cell proliferation and caspase 3/7 activity, and significantly increased the apoptotic rate.

Conclusions: RAD54B exerts an oncogenic role in lung cancer cell proliferation.

摘要：

摘要：

目的:SNF2/SWI2超家族成员RAD54同源B (RAD54B)参与同源重组，RAD54B的高表达可预测肺腺癌的预后。然而，其在肺癌发生中的作用尚不清楚，因此本研究确定了这一点。

方法:采用实时荧光定量PCR、免疫组化染色和western blotting检测RAD54B在15例肺腺癌组织及匹配的邻近健康肺组织中的基因和蛋白表达。用携带小发夹RNA (shRNA)的慢病毒将A549肺癌细胞转导至RAD54B (shRAD54B)或对照shRNA (shCtrl)，观察细胞增殖、活力、凋亡和caspase 3/7活性。

结果:RAD54B蛋白在肺腺癌组织中的表达明显高于正常肺组织。RAD54B基因在A549细胞中表达量较高，但与shCtrl相比，shRAD54B可有效敲除，感染效率为80%，敲除率为72.2%。抑制RAD54B在A549细胞中的表达可显著降低细胞增殖和caspase 3/7活性，并显著提高细胞凋亡率。

结论:RAD54B在肺癌细胞增殖中具有致瘤作用。

该论文中，A549细胞的体外培养是使用Ausbian特级胎牛血清完成的。欲了解或购买Ausbian特级胎牛血清可以联系北京缔一生物400-166-8600.